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assignments:ex10 [2018/10/31 10:13]
dokuroot
assignments:ex10 [2018/11/05 15:14] (current)
dokuroot
Line 22: Line 22:
 **6.** Use ''​Trimmomatic''​ to remove adapter sequences and filter poor quality reads and reads shorter than 16 nt: **6.** Use ''​Trimmomatic''​ to remove adapter sequences and filter poor quality reads and reads shorter than 16 nt:
  
-  $ trimmomatic SE -phred33 ​'​input_file'​ '​output_file'​ ILLUMINACLIP:/​Users/​bz360/​Documents/​Trimmomatic_Files/​TruSeq-smallRNA.fa:​2:​30:​10 MINLEN:16 AVGQUAL:30+  $ trimmomatic SE '​input_file'​ '​output_file'​ ILLUMINACLIP:/​Users/​bz360/​Documents/​Trimmomatic_Files/​TruSeq-smallRNA.fa:​2:​30:​10 MINLEN:16 AVGQUAL:30
  
 What proportion of reads were dropped? What proportion of reads were dropped?
assignments/ex10.1541002387.txt.gz ยท Last modified: 2018/10/31 10:13 by dokuroot