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assignments:ex11 [2017/11/06 11:47]
dokuroot
assignments:ex11 [2017/11/06 11:49] (current)
dokuroot
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 Copy the results into the Excel document and use ''​Excel''​ for performing the normalization in step 4. \\ Copy the results into the Excel document and use ''​Excel''​ for performing the normalization in step 4. \\
  
-**4.** Normalize small RNA reads based on library size (use the value from bowtie, see step 10 from Exercise 10): divide the number of reads for small RNA locus by the total number of reads in millions in each library (reads per million total small RNA reads - RPM). \\+**4.** Normalize small RNA reads based on library size (use the value from bowtie, see step 10 from Exercise 10): divide the number of reads for each small RNA locus by the total number of mapped ​reads in millions in each library (reads per million total small RNA reads - RPM). \\
  
 **5.** Plot the normalized data for each small RNA in ''​Excel''​. \\ \\ **5.** Plot the normalized data for each small RNA in ''​Excel''​. \\ \\
assignments/ex11.txt ยท Last modified: 2017/11/06 11:49 by dokuroot