assignments:ex11

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 assignments:ex11 [2017/11/06 11:47]dokuroot assignments:ex11 [2017/11/06 11:49] (current)dokuroot Both sides previous revision Previous revision 2017/11/06 11:49 dokuroot 2017/11/06 11:47 dokuroot 2017/10/27 14:33 dokuroot 2017/10/27 14:32 dokuroot 2017/10/27 14:32 dokuroot 2017/10/27 14:31 dokuroot 2017/10/27 14:05 dokuroot 2017/10/27 14:05 dokuroot 2017/10/27 14:04 dokuroot 2017/10/27 14:04 dokuroot created 2017/11/06 11:49 dokuroot 2017/11/06 11:47 dokuroot 2017/10/27 14:33 dokuroot 2017/10/27 14:32 dokuroot 2017/10/27 14:32 dokuroot 2017/10/27 14:31 dokuroot 2017/10/27 14:05 dokuroot 2017/10/27 14:05 dokuroot 2017/10/27 14:04 dokuroot 2017/10/27 14:04 dokuroot created Line 28: Line 28: Copy the results into the Excel document and use ''​Excel''​ for performing the normalization in step 4. \\ Copy the results into the Excel document and use ''​Excel''​ for performing the normalization in step 4. \\ - **4.** Normalize small RNA reads based on library size (use the value from bowtie, see step 10 from Exercise 10): divide the number of reads for small RNA locus by the total number of reads in millions in each library (reads per million total small RNA reads - RPM). \\ + **4.** Normalize small RNA reads based on library size (use the value from bowtie, see step 10 from Exercise 10): divide the number of reads for each small RNA locus by the total number of mapped ​reads in millions in each library (reads per million total small RNA reads - RPM). \\ **5.** Plot the normalized data for each small RNA in ''​Excel''​. \\ \\ **5.** Plot the normalized data for each small RNA in ''​Excel''​. \\ \\